Molecular Mechanism of Aflatoxin B₁ Synthesis Related AfVerB Regulating the Development, AFB₁ Biosyntheis and Virulence of <i>Aspergillus flavus</i> Mainly Through Its CYP Domain.

ABSTRACT: Aspergillus flavus and its secondary metabolites aflatoxins pose a significant threat to the health of humans, animals, and plants. Therefore, there is an urgent need to control A. flavus contamination. AfverB plays a key role in the aflatoxin gene cluster; however, its function and mechanism in fungal development and virulence remain poorly understood. In this study, we constructed afVerB gene deletion mutants (∆afVerB-1 and ∆afVerB-2) and two CYP domain mutants (afVerB^∆D1 and afVerB^∆D2) through homologous recombination. Phenotype analysis revealed that, via its two CYP domains, AfVerB is deeply involved in fungal morphogenesis and aflatoxin synthesis. Insect and crop colonization models revealed that AfVerB plays a ke

SUBMITTER: Ye K 

PROVIDER: S-EPMC12028525 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature