Green Extraction Method: Microwave-Assisted Water Extraction Followed by HILIC-HRMS Analysis to Quantify Hydrophilic Compounds in Plants.

ABSTRACT: Background: Hydrophilic compounds, such as amino acids, organic acids and sugars, among others, are present in large amounts in plant cells. The analysis and quantification of these major hydrophilic compounds are particularly relevant in plant science because they have a considerable impact on the quality of plant-derived products and on plant-pathogen relationships. Our objective was to develop and validate a complete analysis workflow combining a water-based extraction procedure with a fast separation using hydrophilic interaction liquid chromatography coupled to high-resolution mass spectrometry (HILIC-HRMS) for quantitative analysis of hydrophilic compounds in plant tissues. Results: Water-based microwave-assisted extraction (MAE) methods for hydrophilic compounds were c

SUBMITTER: Louis A 

PROVIDER: S-EPMC12029008 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature