Production and Characterization of Poly-γ-Glutamic Acid by <i>Bacillus velezensis</i> SDU.

ABSTRACT: In this study, a Bacillus velezensis SDU strain capable of producing poly-γ-glutamate (γ-PGA) was newly identified from the rhizosphere soil of Baimiao taro. The strain is a glutamate-independent strain and can produce polyglutamic acid in a culture medium completely free of glutamate. The hydrolyzed product of the polyglutamic acid produced is D-glutamic acid. The molecular weight of γ-PGA, estimated via the Mark-Houwink equation, was 1390 kDa. Furthermore, the molecular weight measured by Waters gel permeation chromatography with multi-angle laser light scattering (GPC-MALLS) was 1167 kDa. The production of γ-PGA and its antioxidant and tyrosine inhibition properties were investigated. The γ-PGA production reached 23.1 g/L, and the productivity was 0.77 g L^-1 h^-1</

SUBMITTER: Guo G 

PROVIDER: S-EPMC12029172 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature