Barcode-free multiplex plasmid sequencing using Bayesian analysis and nanopore sequencing.

ABSTRACT: Plasmid construction is central to life science research, and sequence verification is arguably its costliest step. Long-read sequencing has emerged as a competitor to Sanger sequencing, with the principal benefit that whole plasmids can be sequenced in a single run. Nevertheless, the current cost of nanopore sequencing is still prohibitive for routine sequencing during plasmid construction. We develop a computational approach termed Simple Algorithm for Very Efficient Multiplexing of Oxford Nanopore Experiments for You (SAVEMONEY) that guides researchers to mix multiple plasmids and subsequently computationally de-mixes the resultant sequences. SAVEMONEY defines optimal mixtures in a pre-survey step, and following sequencing, executes a post-analysis workflow involving sequence classifica

SUBMITTER: Uematsu M 

PROVIDER: S-EPMC12029211 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature