Major nuclear locales define nuclear genome organization and function beyond A and B compartments.

ABSTRACT: Models of nuclear genome organization often propose a binary division into active versus inactive compartments yet typically overlook nuclear bodies. Here, we integrated analysis of sequencing and image-based data to compare genome organization in four human cell types relative to three different nuclear locales: the nuclear lamina, nuclear speckles, and nucleoli. Although gene expression correlates mostly with nuclear speckle proximity, DNA replication timing correlates with proximity to multiple nuclear locales. Speckle attachment regions emerge as DNA replication initiation zones whose replication timing and gene composition vary with their attachment frequency. Most facultative LADs retain a partially repressed state as iLADs, despite their positioning in the nuclear interior. Knock ou

SUBMITTER: Gholamalamdari O 

PROVIDER: S-EPMC12029212 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature