Specific Aspects of SELEX Protocol: Different Approaches for ssDNA Generation.

ABSTRACT:

Background

Synthetic DNA aptamers are a class of molecules with potential applications in medicine, serving as molecular sensors or ligands for targeted drug delivery. Systematic evolution of ligands by exponential enrichment (SELEX) is a technology for selecting functional aptamers that was first reported three decades ago and has been actively developed since. SELEX involves multiple iterations of two fundamental steps: (i) target affinity-based partitioning of aptamers from a random library and (ii) amplification of selected aptamers by PCR, followed by isolation of single-stranded DNA (ssDNA). SELEX protocols have diversified considerably, with numerous variations possible for each step. This heterogeneity makes it challenging to identify optimal methods. Comparative analysis o

SUBMITTER: Garanin A 

PROVIDER: S-EPMC12029403 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature