Development and Evaluation of a MinION Full-Length 16S rDNA Sequencing Analysis Pipeline for Rapid Diagnosis of Animal Gastrointestinal Diseases.

ABSTRACT: Rapid and accurate detection of the causes of gastrointestinal diseases in farmed and companion animals is crucial for advancing livestock farming and safeguarding public health safety. Diseases caused by pathogenic bacteria infections often result in the overrepresentation of pathogens in the gut microbiota; however, gut microbiota dysbiosis without obvious pathogen overrepresentation can also lead to disorders such as inflammatory bowel disease (IBD). Traditional cultivation-based diagnostic methods are time-consuming and ineffective in identifying microbiota dysbiosis-associated diseases. In this study, we developed a sample-to-answer MinION full-length 16S rDNA sequencing analysis pipeline, accompanied by detailed bioinformatics scripts, for the rapid diagnosis of animal gastrointestin

SUBMITTER: Zhong Y 

PROVIDER: S-EPMC12029435 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature