First Detection of Cryptosporidium Canis and Occurrence of <i>Cryptosporidium</i> spp. in Hospitalized Patients in Romania.

ABSTRACT: The study aimed to identify Cryptosporidium infection in hospitalized patients in Western Romania. Stool samples were collected from 175 patients, both male and female, aged between 2 months and 88 years, residing in urban and rural areas. The identification of Cryptosporidium was performed using a qualitative chromatographic rapid test, supplemented by the modified Ziehl-Neelsen method described by Henricksen and Pohlenz. Cryptosporidium spp. were identified through PCR analysis and Sanger sequencing. To assess potential risk factors for cryptosporidiosis, a questionnaire was administered to the study participants. Laboratory test results revealed a cryptosporidiosis occurrence of 7.42%, with a significantly higher occurrence observed in urban areas (p < 0.05).

SUBMITTER: Darabus RG 

PROVIDER: S-EPMC12029450 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature