Genome-Informed Real-Time PCR Assay for Detection of '<i>Candidatus</i> Phytoplasma Prunorum,' Which Is Associated with European Stone Fruit Yellows.

ABSTRACT: 'Candidatus Phytoplasma prunorum' has been associated with severe disease in Prunus spp., which are commodities of economic importance in the USA. The introduction and establishment of 'Ca. P. prunorum' in the USA could result in huge economic losses, thus creating a need for validated diagnostic tools, which are the cornerstone of successful surveillance, quarantine, and eradication measures. Whole-genome comparisons led to the identification of a diagnostic marker gene specific to 'Ca. P. prunorum' (PE639). The PE639 assay was duplexed with an 18S rDNA plant internal control and compared to modified 23S (phytoplasmas) and imp ('Ca. P. mali') assays. The PE639 assay produced congruent results to 23S and imp assays for all metrics, demonstra

SUBMITTER: Yasuhara-Bell J 

PROVIDER: S-EPMC12029454 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature