Evaluation and Application of the MIRA-qPCR Method for Rapid Detection of Norovirus Genogroup II in Shellfish.

ABSTRACT: Globally, norovirus has become the primary cause of outbreaks of acute gastroenteritis, and an increasing number of norovirus GII infections have been associated with shellfish. This highlights the urgent need to establish sensitive and rapid detection platforms for timely screening of contaminated shellfish to reduce the risk of virus transmission. To address this challenge, we developed a novel detection method combining multienzyme isothermal rapid amplification (MIRA) with qPCR, referred to as MIRA-qPCR, specifically targeting norovirus GII. It exhibited robust specificity, demonstrating no cross-reactivity with sapovirus, rotavirus, hepatitis A virus, Escherichia coli, Listeria monocytogenes, or Vibrio parahaemolyticus, and exhibited high sensitivity

SUBMITTER: Zhu Y 

PROVIDER: S-EPMC12029516 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature