Influence of Amino Acids on Quorum Sensing-Related Pathways in <i>Pseudomonas aeruginosa</i> PAO1: Insights from the GEM iJD1249.

ABSTRACT:

Background/objectives

Amino acids (AAs) play a critical role in diseases such as cystic fibrosis where Pseudomonas aeruginosa PAO1 adapts its metabolism in response to host-derived nutrients. The adaptation influences virulence and complicates antibiotic treatment mainly for the antimicrobial resistance context. D- and L-AAs have been analyzed for their impact on quorum sensing (QS), a mechanism that regulates virulence factors. This research aimed to reconstruct the genome-scale metabolic model (GEM) of P. aeruginosa PAO1 to investigate the metabolic roles of D- and L-AAs in QS-related pathways.

Methods

The updated GEM, iJD1249, was reconstructed by using protocols to integrate data from previous models and refined with well-standardized in silico media (LB, M

SUBMITTER: Delgado-Nungaray JA 

PROVIDER: S-EPMC12029727 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature