Exploring the Interplay of Explant Origin and Culture Density on Olive Micropropagation Efficiency.

ABSTRACT: Apical dominance and culture heterogeneity significantly limit the efficiency of olive micropropagation, hindering the rapid production of plantlets. This study explores how manipulating the explant origin (topophysis) and density can mitigate these challenges. Explants originating from apical and middle sections were cultivated at densities of 18, 24, and 30 explants per vessel. After 12 weeks, significant differences in the growth parameters were observed based on the explant origin and density. The middle-section explants exhibited superior shoot proliferation and node production, especially at higher densities. The callus weight also increased with the density, while the internode length remained relatively stable. Hormone analysis demonstrated the density-dependent spatial distributio

SUBMITTER: Grira M 

PROVIDER: S-EPMC12030091 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature