A Convenient Strategy for Studying Antibody Aggregation and Inhibition of Aggregation: Characterization and Simulation.

ABSTRACT: Background/Objectives: Protein aggregation, particularly the aggregation of antibody-based drugs, has long been a significant challenge in downstream processes and formulation. While the inhibitory effects of excipients on aggregation have been extensively studied using early experimental characterization methods, complete formulation research requires significant amounts of antibodies and time, resulting in high research costs. Methods: This study proposed a quick and small-scale position-restrained simulation method which elucidated the mechanism of the reversible self-association (RSA) of antibodies and the influence of excipients on RSA under different conditions. We also validated the rationality of rapid and small-scale simulations through long-term (>1 μs) and large-sc

SUBMITTER: Guo Y 

PROVIDER: S-EPMC12030238 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature