Inhibition of GSK-3β Restores Differentiation Potential of Late-Passage Mesenchymal Stem Cells.

ABSTRACT: Background/Objectives: Mesenchymal stem cells (MSCs) are regarded as a promising cell type with significant therapeutic benefits owing to their ease of isolation, maintenance, and characterisation. However, repeated passages during cultural maintenance frequently result in cellular senescence, limiting their utility in regenerative medicine. Methods: We investigated the differentiation capability between early- (P3) and late-passage MSCs (>P15) and tested the potential of Wnt agonist 99021 to reverse MSCs using standard cell culture protocols that define minimal criteria for MSCs, primarily tri-lineage differentiation assays, biochemical staining gene expression analysis, and senescence assays. Results: We initially noticed distinct signs of morphological aging between

SUBMITTER: Govarthanan K 

PROVIDER: S-EPMC12030495 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature