First Isolation and Characterization of Three Strains of Porcine Sapelovirus in Yunnan Province, China.

ABSTRACT: In order to investigate the causes of swine diarrhea in Yunnan Province, this study was conducted to detect and monitor diarrhea viruses through regular sampling and reverse transcription polymerase chain reaction (RT-PCR). In October 2023, porcine sapelovirus (PSV) was detected in fecal specimens collected from diarrheal pigs in Honghe City, and three strains of PSV were successfully isolated by inoculating them into PK-15 cells; electron microscopy revealed virus particles with diameters of ~32 nm. Next-generation sequencing (NGS) revealed that the PSV isolate genomes ranged from 7480 to 7515 nucleotides in length. Homology analyses indicated that ML-15 and ML-16 showed the highest nucleotide and amino acid identities with the Asian PSV strains, ML-19 showed the highest sequence identiti

SUBMITTER: Zhu P 

PROVIDER: S-EPMC12030907 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature