Microglia Exhibit a Unique Intact HIV Reservoir in Human Postmortem Brain Tissue.

ABSTRACT: A proviral reservoir persists within the central nervous system (CNS) of people with HIV, but its characteristics remain poorly understood. Research has primarily focused on cerebrospinal fluid (CSF), as acquiring brain tissue is challenging. We examined size, cellular tropism, and infection-dynamics of the viral reservoir in post-mortem brain tissue from five individuals on and off antiretroviral therapy (ART) across three brain regions. Microglia-enriched fractions (CD11b⁺) were isolated and levels of intact proviral DNA were quantified (IPDA). Full-length envelope reporter viruses were generated and characterized in CD4⁺ T cells and monocyte-derived microglia. HIV DNA was observed in microglia-enriched fractions of all individuals, but intact proviruses were identi

SUBMITTER: Nuhn MM 

PROVIDER: S-EPMC12030925 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature