Insights from Metabolomic and Transcriptomic Analyses into Sulforaphane's Protective Mechanism Against Deoxynivalenol Toxicity via Spermine Regulation.

ABSTRACT: Deoxynivalenol (DON) is a mycotoxin ubiquitously present in the environment. Emerging evidence demonstrated that sulforaphane (SFN) exerts potent protective effects against DON-triggered cytotoxicity through multimodal mechanisms. This study aimed to investigate the protective mechanism of SFN during DON exposure. Untargeted metabolomics of IPEC-J2 cells revealed a total of 399 differential metabolites between the DON and control group and 365 differential metabolites between the SFN + DON and DON group. KEGG enrichment was performed to investigate the potential regulatory pathways. The transcriptome identified a total of 1839 differential expression genes (DEGs) between DON and SFN + DON groups. This result indicated that DON exposure and SFN treatment have a profound impact on cellular m

SUBMITTER: Xiao Y 

PROVIDER: S-EPMC12031357 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature