Enhancing the Efficacy of Melanoma Treatment: The In Vitro Chemosensitising Impact of <i>Vipera ammodytes</i> Venom on Human Melanoma Cell Lines.

ABSTRACT: Research on viper venom has expanded into diverse medical applications, including cancer treatment. This study investigates the potential of Vipera ammodytes venom in oncology, evaluating its cytotoxicity and chemosensitising effects on malignant melanoma cells. Proteomic analysis identified 125 proteins in the venom, with Phospholipases A2, C-type lectins, and metalloproteinases among the most abundant components. These proteins are associated with cytotoxic, anti-proliferative, and tumor-inhibiting properties. Three melanoma cell lines (M001, Me501, and A375) were used to assess venom cytotoxicity. The IC50 values demonstrated consistent venom sensitivity across cell lines (approximately 1.1 µg/mL). Combined treatment with venom and cisplatin significantly increased the cytotoxici

SUBMITTER: Paolino G 

PROVIDER: S-EPMC12031473 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature