Plant-Derived UDP-Glycosyltransferases for Glycosylation-Mediated Detoxification of Deoxynivalenol: Enzyme Discovery, Characterization, and In Vivo Resistance Assessment.

ABSTRACT: Fungal infections of crops pose a threat to global agriculture. Fungi of the genus Fusarium cause widespread diseases in cereal crops. Fusarium graminearum reduces yields and produces harmful mycotoxins such as deoxynivalenol (DON). Plants mitigate DON toxicity through glucose conjugation mediated by UDP-glycosyltransferases (UGTs), forming deoxynivalenol-3-O-glucoside (DON-3-Glc). Few such UGTs have been identified, predominantly from Fusarium-susceptible crops. Given that the presence of this activity in diverse plants and across broader UGT subfamilies and groups was underexplored, we screened a library of 380 recombinant plant UGTs and identified and characterized eight novel enzymes glycosylating DON in vitro. Among these, ZjUGT from Ziziphus juju

SUBMITTER: Della Gala V 

PROVIDER: S-EPMC12031568 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature