Interaction of Mycotoxins with α₁-Acid Glycoprotein (AGP) and Bovine Milk Proteins: Zearalenone, Zearalenols, and Sterigmatocystin Form Highly Stable Complexes with AGP.

ABSTRACT: Mycotoxins are frequent food contaminants posing health risk to humans and animals. Since these interactions have been barely studied yet, we examined the potential complex formation of mycotoxins with human α₁-acid glycoprotein (AGP) and with bovine milk proteins (including casein (CSN), β-lactoglobulin (LG), and α-lactalbumin (LA)) based on fluorescence spectroscopic and ultracentrifugation techniques. Only weak interactions (logK = 2.7 to 3.5) of certain mycotoxins were observed with CSN, LG, and/or LA. Ultracentrifugation experiments demonstrated that aflatoxin M1, zearalenone, and α-zearalenol form more stable complexes with CSN than with LG or LA. These mycotoxins bound to bovine serum albumin with more than a tenfold higher affinity compared to CSN; nevertheless, i

SUBMITTER: Poor M 

PROVIDER: S-EPMC12031583 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature