Impact of soft tissue homogenization methods on RNA quality.

ABSTRACT:

Background

High-quality RNA isolation from tissues is crucial for transcriptomic analysis. The tissue disruption influences the RNA quality. The aim of this study was to compare different methods of tissue homogenization.

Methods

Three homogenization methods were used (mortar and pestle, ball mill, and tissue homogenizer) to disrupt head and neck cancerous tissues, healthy tissues from free margin of head and neck cancer patients, and breast skin from breast cancer patients. The comparison of isolated RNA quantity and quality by measuring the concentration and absorbance, RIN, and Ct values of reference genes were performed.

Results

RNA isolated after tissue homogenizer usage has the highest 260/230 ratio (p = 0.02) and concentration (p = 0.02) also RIN values tend to

SUBMITTER: Ostapowicz J 

PROVIDER: S-EPMC12031802 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature