A Rhodamine-Based Ratiometric Fluorescent Sensor for Dual-Channel Visible and Near-Infrared Emission Detection of NAD(P)H in Living Cells and Fruit Fly Larvae.

ABSTRACT: The detection and dynamic monitoring of intracellular NAD(P)H concentrations are crucial for comprehending cellular metabolism, redox biology, and their roles in various physiological and pathological processes. To address this need, we introduce sensor A, a near-infrared ratiometric fluorescent sensor for real-time, quantitative imaging of NAD(P)H fluctuations in live cells. Sensor A combines a 3-quinolinium electron-deficient acceptor with a near-infrared rhodamine dye, offering high sensitivity and specificity for NAD(P)H with superior photophysical properties. In its unbound state, sensor A emits strongly at 650 nm and weakly at 465 nm upon 400 nm excitation. Upon binding to NAD(P)H, it shows a fluorescence increase at 465 nm and a decrease at 650 nm, enabling accu

SUBMITTER: Lanquaye H 

PROVIDER: S-EPMC12032585 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature