Two-directional trafficking of the IFT25 protein in the developing mouse sperm flagella.

ABSTRACT: Intraflagellar transport 25 is a component of the intraflagellar transport 25-B complex. In mice, even though this intraflagellar transport component is not required for cilia formation in somatic cells, it is essential for sperm formation. However, the intracellular localization of this protein in male germ cells is not known given no reliable antibodies are available for histologic studies, and the dynamic trafficking in the developing sperm flagella is not clear. To examine localization of the protein in male germ cells and further investigate the mechanism of intraflagellar transport in sperm formation, particularly to look into the dynamic trafficking of the protein, we generated a mouse intraflagellar transport 25-green fluorescent protein knock-in mouse model using the clustered reg

SUBMITTER: Li W 

PROVIDER: S-EPMC12032603 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature