Identification and validation of reference genes with stable expression under elicitor treatments of the medicinal plant Arnica montana L.

ABSTRACT:

Background

In view of enhancing secondary metabolites biosynthesis in Arnica montana through elicitation, comprehensive studies are needed to fully understand the molecular background of biosynthetic pathways in this species. Analysis of transcriptional changes via RT-qPCR technique might shed light on the molecular mechanisms underlying plant reaction to elicitors. This study aimed to identify reference genes which are stably expressed in Arnica under methyl jasmonate, salicylic acid, and yeast extract treatment to provide the basis for current and future gene expression studies in this important medicinal plant.

Results

The expression stability of nine candidate reference genes was evaluated using four widely used algorithms (geNorm, NormFinder, BestKeeper, and ΔCt method)

SUBMITTER: Sozoniuk M 

PROVIDER: S-EPMC12032789 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature