Dynamic changes in lactate-related genes in microglia and their role in immune cell interactions after ischemic stroke.

ABSTRACT:

Objectives

This study aims to elucidate the dynamic changes in lactate-related genes (LRGs) in microglia following ischemic stroke (IS) and their associations with immune cells.

Methods

We performed differential expression analysis on bulk-sequencing (GSE30655 and GSE35338) and scRNA-seq data (GSE174574) to identify differentially expressed genes. These genes were intersected with lactate genes from MSigDB to identify post-stroke LRGs. We used t-SNE to visualize LRG distribution across cell types and selected microglia for cell-cell communication, pseudo time, and functional enrichment analyses. These findings were integrated with the GSE225948 scRNA-seq dataset to examine LRG trends in the chronic phase of IS. Finally, CIBERSORT was used to explore immune cell infiltration

SUBMITTER: Yao J 

PROVIDER: S-EPMC12032981 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature