A novel thermostable lytic phage vB_EF_Enf3_CCASU-2024-3 against clinical Enterococcus faecium and Enterococcus faecalis.

ABSTRACT: Enterococci, Gram-positive bacteria, have become a major concern in healthcare settings due to their significant virulence and antibiotic resistance. This research focuses on isolating, phenotypic, and genotypic analysis of enterococci-specific lytic phages to be used as potential candidates in combating multidrug-resistant (MDR) Enterococcus clinical isolates. The virulence of Enterococcus isolates was analyzed by testing for gelatinase and biofilm formation. The phage(s) was isolated from a sewage sample, then purified, propagated, and physiochemically analyzed. The phage was examined using transmission electron microscopy, and the whole genome sequence (WGS) was performed. Sixety-five clinical enterococci including, 27 (41.5%), 33 (50.7%) 3 (4.6%), and 2 (3%) E. faecalis, E. faecium, E.

SUBMITTER: Amr RM 

PROVIDER: S-EPMC12033158 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature