Calciprotein particle-activated endothelial cells aggravate smooth muscle cell calcification via paracrine signalling.

ABSTRACT:

Background

Vascular calcification is highly prevalent in Chronic Kidney Disease (CKD) and is associated with markedly increased cardiovascular risk. High serum phosphate in CKD increases calcification propensity via generation of circulating calciprotein particles (CPP2), crystalline nanoaggregates composed of calcium, phosphate, and serum proteins. CPP2 induce vascular calcification in vascular smooth muscle cells (VSMCs) in vitro. In vivo, endothelial cells, rather than VSMCs are primarily exposed to CPP2, yet understanding the influence of endothelial cells on vascular calcification is limited.

Methods

We investigated calcification-promoting signalling by endothelial cells on VSMCs. Effects of CPP2 exposure to endothelial cells on CPP2 uptake, endothelial cell activation,

SUBMITTER: Feenstra L 

PROVIDER: S-EPMC12033162 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature