A novel XbaI multiplex PCR method for rapid typing of Klebsiella pneumoniae strains.

ABSTRACT: This study focused on exploiting single-nucleotide polymorphism (SNP) variations in and around XbaI-restriction sites (5'….T↓CTAGA….3') within the genomes of Klebsiella pneumoniae to develop a multiplex PCR genotyping technique that integrates the high discrimination of pulsed-field gel electrophoresis (PFGE) with the straightforwardness of a multiplex PCR-based procedure, for routine use in clinical laboratories. The XbaI-multiplex PCR method was evaluated in silico using 10 reference strains and subsequently compared to PFGE and MLST, revealing similar clustering patterns of test strains in most cases. Furthermore, 29 clinical isolates with known sequence types (STs) were analysed ed using XbaI-multiplex PCR, which demonstrated acceptable discriminative ability and relative concordance w

SUBMITTER: Al-Zahrani IA 

PROVIDER: S-EPMC12033266 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature