Splicing factor PTBP1 promotes hepatocarcinogenesis via oncogenic splice-switching of MAPT.

ABSTRACT:

Background

Alterations in splicing factors contribute to aberrant alternative splicing (AS), which subsequently promotes tumor progression. The splicing factor polypyrimidine tract binding protein 1 (PTBP1) has been shown to facilitate cancer progression by modulating oncogenic variants. However, its specific role and underlying mechanisms in hepatocellular carcinoma (HCC) remain to be elucidated.

Methods

PTBP1 expression was evaluated in HCC tissues and cell lines. Subsequently, cells were transfected with vectors designed for PTBP1 overexpression or downregulation. The biological function of PTBP1 was assessed in vitro and in vivo using MTS assays, colony formation assays, transwell assays, xenograft formation, tail vein injection, and orthotopic models. Tran

SUBMITTER: Zheng W 

PROVIDER: S-EPMC12034000 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature