Bimodal specificity of TF-DNA recognition in embryonic stem cells.

ABSTRACT: Transcription factors (TFs) bind genomic DNA regulating gene expression and developmental programs in embryonic stem cells (ESCs). Even though comprehensive genome-wide molecular maps for TF-DNA binding are experimentally available for key pluripotency-associated TFs, the understanding of molecular design principles responsible for TF-DNA recognition remains incomplete. Here, we show that binding preferences of key pluripotency TFs, such as Pou5f1 (Oct4), Smad1, Otx2, Srf, and Nanog, exhibit bimodality in the local GC-content distribution. Sequence-dependent binding specificity of these TFs is distributed across three major contributions. First, local GC-content is dominant in high-GC-content regions. Second, recognition of specific k-mers is predominant in low-GC-content regions. Third, s

SUBMITTER: Povolotskii M 

PROVIDER: S-EPMC12034040 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature