Zinc sulfate acts as an efflux pump inhibitor on Pseudomonas aeruginosa clinical isolates.

ABSTRACT: Efflux pumps in Pseudomonas aeruginosa play an important role in decreasing the bacterium's antibiotic susceptibility. This study aimed to identify P. aeruginosa efflux pumps and examine the effect of Zinc sulfate on these pumps. This study was conducted on 104 P. aeruginosa clinical isolates collected from different types of specimens. The antimicrobial susceptibility pattern of all isolates was examined by disc diffusion and microdilution methods. A Cartwheel test was done for phenotypic detection of P. aeruginosa efflux pumps in the presence and absence of zinc sulfate. The expression of efflux pumps encoding genes and their regulators in the presence and absence of zinc sulfate was measured using real-time reverse transcriptase PCR. A high rate of multidrug-resistant (MDR) pattern was

SUBMITTER: Abdelraheem WM 

PROVIDER: S-EPMC12034582 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature