Identification of characteristic genes in cutaneous squamous cell carcinoma based on weighted gene co-expression network analysis.

ABSTRACT:

Objective

This study aims to identify characteristic genes associated with cutaneous squamous cell carcinoma (cSCC).

Methods

Differentially expressed genes (DEGs) and hub genes in key module were identified using the limma package and weighted gene co-expression network analysis (WGCNA) in R software, respectively. The intersection of these genes was then subjected to LASSO regression to pinpoint characteristic genes. The correlation between immune cell infiltration and these characteristic genes was further elucidated using single-sample Gene Set Enrichment Analysis and Spearman correlation analysis.

Results

A total of 113 DEGs were identified, along with their associated biological pathways. From this pool, five characteristic genes-ADH1B, CCL27, ID

SUBMITTER: Xiao Q 

PROVIDER: S-EPMC12034687 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature