Lipopolysaccharide preconditioning augments the antibacterial activity of renal macrophages and ameliorates acute kidney injury caused by <i>Staphylococcus aureus</i> bacteremia in mice.

ABSTRACT: IntroductionRepeated injections of low-dose lipopolysaccharide (LPS preconditioning) augment the antibacterial activity of liver macrophages. In this study, a mouse model of acute kidney injury (AKI) induced by Staphylococcus aureus (S. aureus) bacteremia was used to investigate the effects of LPS preconditioning on renal macrophages.MethodsEight-week-old C57BL/6J mice were preconditioned with either low-dose LPS (5 μg/kg) or the vehicle for three consecutive days. Kidney immune cells were isolated, and the antibacterial activity of renal macrophages was assessed by pHrodo^TM-labeled S. aureus in vitro. Twenty-four hours after the last LPS injection, the mice were intravenously challenged with S. aureus (2 × 10⁷ CFU) and their renal function was ev

SUBMITTER: Tanoue K 

PROVIDER: S-EPMC12035103 | biostudies-literature | 2025 Jan-Dec

REPOSITORIES: biostudies-literature