<i>In situ</i> mass spectrometry imaging reveals metabolite alterations in gray and white matter after spinal cord injury.

ABSTRACT: It is known that spinal cord injury (SCI) causes metabolic disorders, such as disrupted lipid and amino acid metabolism. However, the in situ changes of metabolites in the spinal cord remain elusive. In the present study, adult Sprague-Dawley rats underwent spinal cord transection surgery. Ambient air flow-assisted desorption electrospray ionization mass spectrometry imaging was performed to identify the localization of metabolites in the white and gray matter and the ventral and dorsal horns within the spinal cord sections. The results revealed that 42 metabolites were specifically increased in the gray matter, and 90 metabolites were increased in the white matter compared with that in the sham group. In the ventral and dorsal horns, 86 and 103 metabolites, respectively, exhibited

SUBMITTER: Lyu C 

PROVIDER: S-EPMC12035510 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature