Crystallization-Assisted Asymmetric Synthesis of Enantiopure Amines Using Membrane-Immobilized Transaminase.

ABSTRACT: The production of active pharmaceutical ingredients (APIs) requires enantiopure chiral amines, for which greener synthesis processes are needed. Transaminases (TAs) are enzymes that catalyze the enantioselective production of chiral amines from prochiral ketones through transamination under mild conditions. Yet, industrial applications of biocatalytic transamination remain currently hindered by the limited stability of soluble enzymes and by the unfavorable thermodynamic equilibrium of targeted asymmetric reactions. Enzyme immobilization can be applied to address stability, recoverability, and reusability issues. In the perspective of process intensification, we chose to immobilize TAs on polymeric (polypropylene) membranes. In the asymmetric synthesis of (R)-2-fluoro-α-methylbenzylamine (

SUBMITTER: Meersseman Arango H 

PROVIDER: S-EPMC12035565 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature