Deciphering of intra-tumoural heterogeneity and the interplay between metastasis-associated meta-program and myofibroblasts in gastric cancer.

ABSTRACT:

Background

Gastric cancer (GC) exhibits high heterogeneity that relies on the oncogenic properties of cancer cells and multicellular interactions in the tumour microenvironment. However, the heterogeneity of GC and their molecular characteristics are still largely unexplored.

Methods

We employed single-cell and spatial transcriptomics to comprehensively map the intra-tumoural heterogeneity within GC. Additionally, in vitro experiments, clinical sample analyses, and patient-derived organoid models (PDOs) were conducted to validate the key interaction patterns between tumor cells and stromal cells.

Results

Seven robust meta-programs (MP1-MP7) in GC were defined with distinct biological significance and spatial distributions. MP3 and MP4 were intimately associated with d

SUBMITTER: Wu X 

PROVIDER: S-EPMC12035649 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature