Efficient production of 2'-fucosyllactose in <i>Pichia pastoris</i> through metabolic engineering and constructing an orthogonal energy supply system.

ABSTRACT: 2'-fucosyllactose (2'-FL) holds significant role in the infants' nutrition. While microbial production of 2'-FL has predominantly utilized Escherichia coli and Saccharomyces cerevisiae, the potential of Pichia pastoris, renowned for its robust NADPH regeneration capability, remains underexplored. Herein, we systematically engineered the metabolism of P. pastoris to develop an efficient 2'-FL-producing cell factory. We first constructed the de novo biosynthesis pathway for 2'-FL in P. pastoris, achieving an initial titer of 0.143 g/L. By optimizing enzyme selection and solubility of α-1,2-fucosyltransferase (FutC), 2'-FL production was enhanced by nearly ten folds. Subsequently, engineering NADPH supply further increased the 2'-FL production by 170 

SUBMITTER: Li Y 

PROVIDER: S-EPMC12035725 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature