Reactions of [13C]-labelled tobacco smoke with DNA to generate selected adducts formed without metabolic activation.

ABSTRACT: DNA adducts are central in the carcinogenic process because they can cause miscoding leading to permanent mutations in important genes involved in carcinogenesis. While it is known that tobacco smoking leads to increased levels of multiple DNA adducts, most DNA adducts detected to date in humans cannot be explicitly attributed to smoking but instead have various possible exogenous and endogenous sources. We plan to probe the tobacco source of DNA adducts by providing carbon-13 labelled ([13C]-labelled) cigarettes to smokers and analyzing [13C]-labelled DNA adducts in their oral cells to determine which adducts arise from smoking. Prior to conducting studies in humans, we first report here proof-of-principle machine smoking experiments to evaluate carbon isotopologues of (a) selected carbon

SUBMITTER: Tang MK 

PROVIDER: S-EPMC12035816 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature