Greening the Production of Indigo Blue Exploiting Light and a Recombinant Synechocystis sp. PCC6803 Strain Expressing the Enzyme mFMO.

ABSTRACT: Cyanobacteria are emerging as interesting cell factories, offering the significant advantage of their in-built photosynthetic machinery, which generates NADPH to support redox biocatalysis. In this study, we assessed the potential of the cyanobacterium Synechocystis sp. PCC6803 in producing the dye indigo by light-driven whole-cell biotransformation using indole as a starting compound. A stable transgenic strain expressing a flavin-containing monooxygenase from Methylophaga aminisulfidivorans (mFMO) was engineered, enabling light-dependent indigo production. Upon optimising conditions, effective biotransformations could be performed, resulting in 112 mg/L indigo (86% conversion of the furnished indole). Additionally, we present a method for the recovery of the secreted dye directly from th

SUBMITTER: Loprete G 

PROVIDER: S-EPMC12035870 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature