WRAP-based nanoparticles for siRNA delivery in zebrafish embryos by simple bath immersion.

ABSTRACT: The use of RNA interference (RNAi) is becoming more widespread in several areas of biomedical research. However, the success of RNAi depends on the effective delivery of siRNA in vitro or in vivo. Efforts are under way to identify universally effective delivery systems. Promising candidates include cell-penetrating peptides, such as the WRAP (tryptophan and arginine-rich amphipathic peptide) family, which forms nanoparticles in the presence of short interfering RNA (siRNA). Here, we optimized the WRAP-based nanoparticles for zebrafish embryo transfection by first determining the ideal formulation compatible with the saline solution required for zebrafish embryo care. We found that adding 20% polyethylene glycol (PEG) to the WRAP1 nanoparticles provided the best nanoparticles

SUBMITTER: Konate K 

PROVIDER: S-EPMC12036052 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature