Streptococcus mutans-derived extracellular vesicles promote skin wound healing via tRNA cargo.

ABSTRACT:

Background

The human oral cavity harbors a diverse microbiota, including Streptococcus species. Oral mucosal wounds heal rapidly, although the exact cause remains unclear. This study investigates the impact of Streptococcus mutans-derived extracellular vesicles (Sm EVs) on wound healing in both oral mucosal organoids and mouse skin. To explore whether microbial EV RNA cargo influences wound healing, RNA sequences from Sm EVs were identified, and the most abundant sequences were synthesized into oligomers and encapsulated in E. coli EVs (Ec EVs) for further in vivo testing. We assessed the role of Toll-like receptor 3 (TLR3) in the wound healing mechanism in TLR3 knockout (KO) mice.

Results

Sm EVs significantly enhanced cell proliferation and migration in oral mucosa, with en

SUBMITTER: Oh SY 

PROVIDER: S-EPMC12036164 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature