Comparative evaluation of five β-Lactamase inhibitors in combination with β-Lactams against multidrug-resistant Mycobacterium tuberculosis in vitro.

ABSTRACT:

Objective

Evaluating the activity of six β-lactams in combination with different β-lactamase inhibitors to identify the most potent combination against Mycobacterium tuberculosis(MTB) in vitro.

Methods

A total of 105 MDR-TB strains from different regions of Henan province were included in this study.Drug susceptibility of sixβ-lactams alone or in combination with β-lactamase inhibitors was examined by broth dilution method against 105 clinical isolates.Mutations of blaC, ldt_mt1,dacB2and ldt_mt2 were analyzed by PCR and DNA sequencing.

Results

Out of the β-lactams used herein, tebipenem was the most effective against MDR-TB and had an MIC₉₀ value of 16 µg/ml.Clavulanic acid, tazobactam, and sulbactam, demonstrated the best synergy with t

SUBMITTER: Shi J 

PROVIDER: S-EPMC12036291 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature