<i>Burkholderia cenocepacia</i> and <i>Pseudomonas aeruginosa</i> in dual-species models: Insights into population distribution, antibiotic susceptibility, and virulence.

ABSTRACT: Multispecies biofilms are communities composed of different microorganisms embedded in an auto-synthesized polymeric matrix. Pseudomonas aeruginosa and Burkholderia cenocepacia are two multidrug-resistant and biofilm-forming opportunistic pathogens often found in the lungs of people living with cystic fibrosis. In this context, planktonic, static, and dynamic biofilms and in vivo models of both species were optimized in this work to understand their population dynamics, disposition, virulence, and antibiotic susceptibility. From the coculture models optimized in this work, we determined that B. cenocepacia grows in a clustered, aggregative manner at the bottom layers of biofilms, in close contact with P. aeruginosa, that tends to occupy the top layers. Th

SUBMITTER: Alcacer-Almansa J 

PROVIDER: S-EPMC12036490 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature