Expression and molecular characterization of an intriguing hyaluronan synthase (HAS) from the symbiont "<i>Candidatus</i> Mycoplasma liparidae" in snailfish.

ABSTRACT:

Background

Hyaluronan synthases (HASs) are ubiquitous in living organisms, and the hyaluronic acid (HA) synthesized by them are important to their body and well used in medicine, cosmetics and other fields. HAS from deep-sea creatures has not yet been explored before. The study aims to analyse the characteristics and enzyme kinetics of a novel hyaluronan synthase derived from the symbiont "Candidatus Mycoplasma liparidae" found in deep-sea snailfish (snHAS).

Methodology

snHAS was over-expressed using His ₆ as tag in the study. The sequence alignment was conducted by Cluster W and then the phylogenetic analyse of HASs was performed by Mega 6.0 to investigate the position of snHAS during evolution. K _m and V _max were

SUBMITTER: Guo L 

PROVIDER: S-EPMC12036578 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature