An integrated mechanism of G_q regulation of PLCβ enzymes.

ABSTRACT: Phospholipase Cβ (PLCβ) enzymes are the principal effectors activated by G_q heterotrimers. Both Gα_q and Gβγ subunits can activate PLCβ, which requires precise positioning of PLCβ at the plasma membrane to relieve structural autoinhibition and give the active site access to the phosphatidylinositol 4,5-bisphosphate (PIP2) substrate. PLCβ enzymes possess a unique distal C-terminal domain (dCTD) that is critical for activation by Gα_q, but the reason for this is unclear. It is also not known how G protein activation affects the subcellular localization of PLCβ enzymes, some of which are found primarily in the cytosol despite needing to act at the plasma membrane. Here, we use bioluminescence spectroscopy, imaging, and gene editing to study the membrane disposit

SUBMITTER: Senarath K 

PROVIDER: S-EPMC12037048 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature