Characterization of two GH10 enzymes with ability to hydrolyze pretreated Sorghum bicolor bagasse.

ABSTRACT: In this study, we characterized two novel enzymes of the glycoside hydrolase family 10 (GH10), Xyl10 C and Xyl10E, identified in the termite gut microbiome. The activities of both enzymes were assayed using beechwood xylan, barley β-glucan, and pretreated Sorghum bicolor bagasse (SBB) as substrates. Both enzymes, assessed individually and in combination, showed activity on beechwood xylan and pretreated SBB, whereas Xyl10E also showed activity on barley β-glucan. The composition of pretreated SBB mainly consisted of xylose and arabinose content. Purified Xyl10 C showed optimum xylanase activity in the pH range 7.0-8.0 and at a temperature of 50-60 °C, while Xyl10E was active at a wider pH range (5.0-10.0) and at 50 °C. The residual activities of Xyl10 C and Xyl10E after 8 h of incubation a

SUBMITTER: Bruno Baron C 

PROVIDER: S-EPMC12037437 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature