Identification of a novel chromosome-encoded fosfomycin resistance gene <i>fosC3</i> in <i>Aeromonas caviae</i>.

ABSTRACT:

Background

Owing to the rapid emerging of multidrug-, even pandrug-resistant pathogens, and lack of new antibiotics, the older antibiotic, fosfomycin, has been reused in recent years in the clinical practice, especially for treatment of uropathogen infections. With the increased use of fosfomycin, bacterial resistance to it has also increased drastically. Elucidating the resistance mechanism to the antimicrobial has become an urgent task.

Methods

The putative fosfomycin resistance gene fosC3 was cloned, and minimal inhibitory concentrations were determined by the agar dilution method. Enzyme kinetic parameters were measured by high-performance liquid chromatography. Bioinformatics analysis was applied to understand the evolutionary characteristics of FosC3.

Results

SUBMITTER: Lu J 

PROVIDER: S-EPMC12037509 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature