Custom barcoded primers for influenza A nanopore sequencing: enhanced performance with reduced preparation time.

ABSTRACT: Highly pathogenic avian influenza is endemic and widespread in wild birds and is causing major outbreaks in poultry worldwide and in U.S. dairy cows, with several recent human cases, highlighting the need for reliable and rapid sequencing to track mutations that may facilitate viral replication in different hosts. SNP analysis is a useful molecular epidemiology tool to track outbreaks, but it requires accurate whole-genome sequencing (WGS) with sufficient read depth across all eight segments. In outbreak situations, where timely data is critical for controlling the spread of the virus, reducing sequencing preparation time while maintaining high-quality standards is particularly important. In this study, we optimized a custom barcoded primer strategy for influenza A whole-genome sequencing

SUBMITTER: Goraichuk IV 

PROVIDER: S-EPMC12037546 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature