Lab-in-a-Tip: a multiplex immunoassay platform based on a self-assembled barcoded protein array.

ABSTRACT: High throughput immunoassay is increasingly crucial for both scientific and clinical applications. Here we propose a "Lab-in-a-Tip" (LIT) concept to fabricate a pipette tip containing a high-density protein array and other essential reagents. The protein array is made by self-assembling digitally encoded microparticles inside the modified tip. Mounted on a robotic workstation, it automates liquid-handling steps. Notably, compared with Luminex, the current gold standard in multiplex immunoassays, such a design enables LIT to demonstrate multiple advantages in terms of analytical sensitivity, speed, and throughput. It detects analyte concentrations as low as fg/ml, representing a sensitivity improvement of two orders of magnitude over Luminex. Incubation time is reduced to 15 minutes from Lu

SUBMITTER: He Y 

PROVIDER: S-EPMC12037755 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature