Alternate Grainy head isoforms regulate Drosophila midgut intestinal stem cell differentiation.

ABSTRACT: Regeneration of the Drosophila midgut epithelium depends upon differential expression of transcription factors in intestinal stem cells and their progeny. The grainy head locus produces multiple splice forms that result in production of two classes of transcription factor, designated Grh.O and Grh.N. grainy head expression is associated with epithelial tissue and has roles in epidermal development and regeneration but had not been examined for a function in the midgut epithelium. Here we show that null mutant clones had a limited effect on intestinal stem cell (ISC) maintenance and proliferation but surprisingly specific loss of all Grh.O isoforms results in loss of ISCs from the epithelium. This was confirmed by generation of a new Grh.O class mutant to control for genetic background effe

SUBMITTER: Dominado N 

PROVIDER: S-EPMC12037896 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature